Isolation and characterization of aerobic gut microbiome of psoriatic arthritic and psoriasis patients
Rakesh Sarma
Balanehru Subramanian
Central Interdisciplinary Research Facility, Sri Balaji Vidyapeeth (Deemed University), Pondicherry - 607402
Krishnan Subramanian
Department of Dermatology, Venerology and Leprology, Mahatma Gandhi Medical College and Research Institute, Pondicherry - 607402
PDF

Keywords

Psoriatic arthritis, Psoriasis, Inflammation, Fecal sample, Bacteria, Gut microbiome, Gut microbiota, Puducherry.

How to Cite

1.
Sarma R, Subramanian B, Subramanian K. Isolation and characterization of aerobic gut microbiome of psoriatic arthritic and psoriasis patients. jbcahs [Internet]. 19Feb.2018 [cited 16Dec.2018];2(1):20-4. Available from: https://www.jbcahs.org/journal/article/view/62

Abstract

Background & Objectives: Psoriatic arthritis (PsA) and Psoriasis (Ps) is a chronic inflammatory disorder affecting mainly the skin and synovial membrane. While the etiology remains underexplored, multiple factors seem to play a role in pathogenesis.  Reports show that the gut microbiome contributes to arthritis and related inflammation of the joints.

Methods This study was initiated to understand the variation in the microbiome in PsA patients visiting the Mahatma Gandhi Medical College and Research Institute(MGMCRI) hospital, Puducherry. Fecal samples were collected from 15clinically and diagnostically confirmed as PsA and Ps patients.  They were subjected to standard microbiological analysis.

Results: Gram positive Staphylococcus aureus and Streptococci constituted 30% of the isolates. Remaining 70% included Gram negative bacteria with a predominant presence of Escherichia coli, Enterococcus, Klebsiella, Pseudomonas, and Proteus.

Conclusion: A total of 30 aerobic bacterial isolates were identified from the fecal samples of PsA and Ps Patients visiting MGMCRI hospital. To the best of our knowledge, this is the first South Indian study characterizing the fecal microbial compositions in PsA patients

PDF

References

1. Eppinga H, Konstantinov SR, Peppelenbosch MP, Thio HB. The microbiome and psoriatic arthritis. Curr Rheumatol Rep. 2014; 16:407

2. Wilson FC, Icen M, Crowson CS, McEvoy MT, Gabriel SE, Kremers HM. Time trends in epidemiology and characteristics of psoriatic arthritis over 3 decades: a population-based study. J Rheumatol. 2009; 36:361–7.

3. Christophers E, Barker JN, Griffiths CE, Dauden E, Milligan G, Molta C, et al. The risk of psoriatic arthritis remains constant following initial diagnosis of psoriasis among patients seen in European dermatology clinics. J Eur Acad Dermatol Venereol. 2010; 24:548–54.

4. Ritchlin CT. Pathogenesis of psoriatic arthritis. Curr Opin Rheumatol. 2005; 17:406–12.

5. Konstantinov SR, van der Woude CJ, Peppelenbosch MP. Do pregnancy-related changes in the microbiome stimulate innate immunity? Trends Mol Med. 2013; 19:454–9

6. Jose U. Scher, Carles Ubeda, Alejandro Artacho, Mukundan Attur, Sandrine Isaac, Soumya M. Reddy, et.al. Decreased bacterial diversity characterizes an altered gut microbiota in psoriatic arthritis and resembles dysbiosis of inflammatory boweldDisease. Arthritis Rheumatol.2015; 67: 128–39

7. Cua DJ, Sherlock JP. Autoimmunity's collateral damage: Gut microbiota strikes ‘back'. Nat Med. 2011; 7:1055-6.

8. Van Praet L, Jacques P, Van den Bosch F, Elewaut D. The transition of acute to chronic bowel inflammation in spondyloarthritis. Nat Rev Rheumatol. 2012; 17:288-95.

9. Sherlock JP, Joyce-Shaikh B, Turner SP, Chao CC, Sathe M, Grein J et al. IL-23 induces spondyloarthropathy by acting on ROR-γt+ CD3+CD4-CD8- entheseal resident T cells. Nat Med. 2012; 18:1069-76

10. Van Praet L, Van den Bosch FE, Jacques P, Carron P, Jans L, Colman R et al. Microscopic gut inflammation in axial spondyloarthritis: a multiparametric predictive model. Ann Rheum Dis. 2013; 72:414-7
11. Jose U. Scher,Dan R. Littman, MD, and Steven B. Abramson. Microbiome in inflammatory arthritis and human rheumatic diseases. Arthritis Rheumatol. 2016; 68: 35–45

12. Owen CM, Chalmers RJ, O'Sullivan T, Griffiths CE. Antistreptococcal interventions for guttate and chronic plaque psoriasis. Cochrane Database Syst Rev. 2000;(2):CD001976


13. Yan D, Issa N, Afifi L, Jeon C, Chang HW, Liao W. The Role of the skin and gut microbiome in psoriatic disease. Curr Dermatol Rep. 2017; 6:94-103

14. Moll JMH, Wright V. Psoriatic arthritis. Semin Arthritis Rheum 1973; 3:55-78.

15. Holt, GH; Krieg, NR; Sneath, PHA; Staley, JT and Williams, ST (1994). Bergey’s manual of determinative bacteriology. 9th Edn., Baltimore, Maryland, Williams and Wilkins. P: 787.

16. Kandler, O., Weiss, N., in Sneath, P.H.A., Mair, N.S., Sharpe, M.E. and Holt, J.G. (1986) Bergey’s Manual of Systematic Bacteriology (Eds), 2, Baltimore, Williams and Wilkins, pp. 1209-1234.

17. National Institute for Health and Clinical Excellence. Psoriasis: the management of psoriasis. NICE, 2012. [cited 2017] https://www.nice.org.uk/guidance/cg153

18. Keithlin J, Sargeant JM, Thomas MK, Fazil A. Systematic review and meta-analysis of the proportion of non-typhoidal Salmonella cases that develop chronic sequelae. Epidemiol Infect. 2015; 143:1333-51

19. Keithlin J, Sargeant JM, Thomas MK, Fazil A. Systematic review and meta-analysis of the proportion of non-typhoidal Salmonella cases that develop chronic sequelae. Epidemiol Infect. 2015 ;143:1333-51

20. Dworkin MS, Shoemaker PC, Goldoft MJ, Kobayashi JM. Reactive arthritis and Reiter’s syndrome following an outbreak of gastroenteritis caused by Salmonella enteritidis. Clin Infect Dis.2001; 33:1010–4.

21. Hannu T, Mattila L, Siitonen A, Leirisalo-Repo M. Reactive arthritis attributable to Shigella infection: a clinical and epidemiological nationwide study. Ann Rheum Dis. 2005; 64:594–98

22. Ajene AN, Fischer Walker CL, Black RE. Enteric pathogens and reactive arthritis: a systematic review of Campylobacter, salmonella and Shigella-associated reactive arthritis. J Health Popul Nutr. 2013 ;31:299-07

23. Johnson JR. Shigella and Escherichia coli at the crossroads: Machiavellian masqueraders or taxonomic treachery? J Med Microbiol.2000;49:583–5.

24. Del Porto F, Proietta M, Muscianese M, Tamburi F, Cifani N, Ferri L et al. Granuloma annularis revealing Wegener'sgranulomatosis. Int J Immunopathol Pharmacol. 2014; 27:273-8

25. Ng CY, Huang YH, Chu CF, Wu TC, Liu SH,. Risks for Staphylococcus aureus colonization in patients with psoriasis: a systematic review and meta-analysis. Br J Dermatol. 2017 Oct; 177:967-977

26. Fatima Zahra Elfatoiki, Mohamed El Azhari, Assiya El Kettani, Zineb Serhier, Mohamed Bennani Othmani, Mohamed TiminounI et al. Psoriasis and staphylococcus aureus skin colonization in Moroccan patients. Pan Afr Med J.2016; 23: 33.

27. Ong PY, Ohtake T, Brandt C, Strickland I, Boguniewicz M, Ganz T et al. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. N Engl J Med. 2002; 347:1151–60.

Downloads

Download data is not yet available.